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Herpes Simplex Virus Non-structural Proteins. III. Function of the Major DNA-binding Protein

Edward Littler

Department of Microbiology, University of Leeds, Leeds LS2 9JT, U.K.
and¹ Department of Pathology, University of Cambridge, Laboratories Block, Addenbrooke's Hospital, Hills Road, Cambridge CB1 2QQ, U.K.

The herpes simplex virus type 2 major DNA-binding protein has been functionally characterized using temperature-sensitive mutants in the complementation group 2-2. The mutants were shown to be defective in the DNA-binding protein gene by mapping the mutants to the area of the genome known to code for the protein, and by demonstrating alterations in the major DNA-binding protein induced in mutant-infected cells. The mutants were shown to be defective in the replication of virus DNA. The nature of this defect was examined by studying virus DNA synthesis in vitro and by the examination of virus enzymes. An effect of mutation in the DNA-binding protein was to destabilize both the DNA polymerase and the alkaline exonuclease.

Keywords: HSV, DNA-binding proteins, ts mutants, protein function

Present address: Department of Pathology, McMaster University, Hamilton, Ontario, Canada.

Received 15 November 1982; accepted 30 December 1982.

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