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Baculovirus Replication: Inhibition of Trichoplusia ni Multiple Nuclear Polyhedrosis Virus by [E]-5-(2-Bromovinyl)-2'-deoxyuridine

X. Wang^1,

¹ Natural Environment Research Council, Institute of Virology, Mansfield Road, Oxford OX1 3SR, U.K.
and² Rega Instituut, Katholieke Universiteit, Leuven, Minderbroedersstraat 10, B-3000 Leuven, Belgium

[E]-5-(2-bromovinyl)-2'-deoxyuridine (BVdU) inhibits the replication of the baculovirus Trichoplusia ni multiple nucleocapsid nuclear polyhedrosis virus in Spodoptera frugiperda cells. Virus-specific DNA synthesis and late protein synthesis are suppressed by the drug. BVdU is phosphorylated by deoxythymidine (deoxycytidine) kinase present in both uninfected and virus-infected cells, and in its 5'-triphosphate form it inhibits DNA polymerase activity in virus-infected cells. The effect of the BVdU is not completely reversible. Phosphonoacetic acid, phosphonoformic acid and Acyclovir have no effect on baculovirus replication. Acyclovir fails to compete with deoxycytidine and thymidine as substrates for pyrimidine deoxynucleoside kinase in virus-infected and uninfected cells.

Keywords: baculovirus, NPV replication, BVdU, inhibition

On sabbatical leave from the Institute of Entomology, Zhongshan University, Guangzhou, China.

Received 22 November 1982; accepted 24 January 1983.

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