HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Abraham, G. Articles by Pattnaik, A. K. Search for Related Content PubMed PubMed Citation Articles by Abraham, G. Articles by Pattnaik, A. K. Agricola Articles by Abraham, G. Articles by Pattnaik, A. K.

Early RNA Synthesis in Bunyamwera Virus-infected Cells

School of Science, Griffith University, Nathan, Queensland 4111, Australia

RNA synthesis in Bunyamwera virus-infected cells was analysed either by sedimentation analysis in SDS-containing sucrose gradients or by hybridization procedures involving annealing with viral genome RNA (vRNA) followed by electrophoretic analysis. Using either procedure, none of the virus-specific RNAs from infected cells was found to be polyadenylated when analysed by oligo(dT)-cellulose chromatography. In addition, viral messenger RNA activity was found to be associated only with non-polyadenylated RNA species when assayed in an in vitro translation system. The infected cell RNAs could be partially resolved by sucrose gradient centrifugation, and virus-specific RNAs of each polarity were present in these preparations which indicated that the characteristic amplification of secondary transcription was occurring. In the presence of cycloheximide or puromycin, no detectable primary RNA transcription occurred. The same inhibitors, when used later in the infection cycle, caused a dramatic and almost complete inhibition of secondary RNA transcription. The inhibition of RNA synthesis caused by these drugs appeared to be fully reversible. Thus, these inhibitors of protein synthesis affect both primary and secondary RNA transcription by Bunyamwera virus indicating that this virus employs transcription mechanisms different from those known for other families of negative-stranded viruses. Hybridization of ³²P-labelled vRNA from Bunyamwera virus with RNA extracted from virus-infected cells produced four duplex RNA molecules that were resolved by gel electrophoresis. Analysis by hybridization and oligonucleotide mapping showed that the two larger duplexes contained complementary (c)RNAs that were transcribed from the L and M segments of viral RNA while the cRNAs contained in the two smaller duplexes were both transcribed from the S RNA segment. Based on a comparison of their oligonucleotide fingerprints, the two latter cRNAs showed a considerable sequence overlap.

Keywords: Bunyamwera virus, RNA synthesis, RNA hybridization, oligonucleotide mapping

Received 11 November 1982; accepted 25 January 1983.

This article has been cited by other articles:

				J. N. Barr Bunyavirus mRNA synthesis is coupled to translation to prevent premature transcription termination RNA, May 1, 2007; 13(5): 731 - 736. [Abstract] [Full Text] [PDF]

				D. Kolakofsky, C. Bellocq, and R. Raju The Translational Requirement for La Crosse Virus S-mRNA Synthesis Cold Spring Harb Symp Quant Biol, January 1, 1987; 52(0): 373 - 379. [Abstract] [PDF]