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Quantitative Analysis of the Cooperative Effect between Inducers of Epstein—Barr Virus Antigen Synthesis

Institut für Virologie, Zentrum für Hygiene, Universität Freiburg, Hermann-Herder-Strasse 11, 7800 Freiburg, Federal Republic of Germany

Induction of Epstein—Barr virus antigens in Raji cells by 12-O-tetradecanoylphorbol 13-acetate (TPA), 5'-iodo-2'-deoxyuridine (IUdR), n-butyric acid (n-BA) antibody to IgM (anti-IgM) and a recently described serum factor was studied after single applications of individual inducers or of their combinations. Whereas individual inducers led to activation only in a limited number of cells, certain combinations of inducers greatly enlarged the reactive cell population. In synergistically acting combinations of drugs, the dose necessary for half-maximal induction of the first inducer was shifted to a much lower value by the second inducer. The serum factor cooperated with any of the four inducers TPA, IUdR, anti-IgM and n-butyric acid, whereas TPA only interacted with IUdR and n-butyric acid. The effects of the serum factor and of TPA showed some similarities but were not identical, whereas IUdR and n-BA showed identical effects.

Keywords: EBV, antigen induction, synergistic effect, serum factor

Received 28 October 1982; accepted 12 January 1983.