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The Role of Pseudorabies Virus Thymidine Kinase Expression in Trigeminal Ganglion Infection

Departments of Medicine (Neurology) and Microbiology and Cancer Research Center, The Pennsylvania State University College of Medicine, Hershey, Pennsylvania 17033, U.S.A.

The role of pseudorabies virus (PRV) thymidine kinase (TK) expression in the pathogenesis of PRV infection of mice was studied with TK-negative (TK^-) mutants. Thymidine phosphorylation and arabinosylthymine inhibition of PRV replication and efficiency of plating were used to characterize TK⁺ and TK^- PRV. In addition, a plaque autoradiography procedure was utilized to determine the TK phenotype of individual plaques. TK⁺ and TK^- PRV replicated well in ocular tissues, while TK⁺ but not TK^- did so in ganglion tissue. Mortality was absent after TK^- PRV inoculation and widespread after inoculation of similar amounts of TK⁺ PRV. Latent infection in mice was not detected with either TK⁺ or TK^- PRV. This study indicated the probable importance of PRV TK expression in acute trigeminal ganglion infection.

Keywords: PRV infection, PRV thymidine kinase, trigeminal ganglion infection

Received 26 October 1982; accepted 25 January 1983.