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Properties of Non-glycosylated Human Interferon- from MG63 Cells

Institute for Virus Research, Kyoto University, Kyoto 606, Japan

MG63 cells induced by Sendai virus produce human interferon (IFN)- with a molecular weight of 22000. In the presence of tunicamycin, they produced non-glycosylated, biologically active, IFN- with a molecular weight of 17000. It had slightly lower heat stability (at 56 °C) than normal glycosylated IFN, but the sensitivity to neutralization by antisera and the cross-species activities on murine, bovine and hamster cells were unaltered, indicating dispensability of the carbohydrate moiety for these parameters. However, the IFN yields were markedly reduced by the glycosylation inhibitor, suggesting an important role of glycosylation in production by cells of IFN-.

Keywords: interferon (HuIFN-), glycosylation, heat stability, cross-species activity

Received 28 September 1982; accepted 28 December 1982.

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