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Characterization of Ribonucleic Acid Transcriptase in Ibaraki Virus Core Particles

Department of Virology, The Kitasato Institute, 9-1, Shirokane 5 chome, Minato-ku, Tokyo 108, Japan
and¹ The Azabu University, Sagamihara-shi, Kanagawa 229, Japan

Ibaraki virus core particles were purified from infected BHK-21 cells. The core particles displayed RNA transcriptase activity while the virion did not. The RNA transcriptase activity was stimulated about 15-fold by addition of S-adenosyl-L-methionine. The reaction product was single-stranded RNA which could be hybridized with heat-denatured Ibaraki virus RNA. The hybrids possessed the same electrophoretic mobility as Ibaraki virus double-stranded RNA.

Keywords: orbivirus, Ibaraki virus, core particles, RNA transcriptase

Received 20 October 1982; accepted 8 February 1983.