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Purification and Molecular Weight Determination of Measles Virus Genomic RNA

Institute of Virology and Immunobiology, University of Würzburg, D-8700 Würzburg, Federal Republic of Germany
and¹ Institute of Molecular Biology I, University of Zürich, CH-8093 Zürich, Switzerland

A purification procedure for genomic measles virus RNA, free of contaminating smaller RNA and of DNA, is described. Viral nucleocapsids were prepared from MA160 cells infected in spinner cultures with measles virus (Edmonston strain). Nucleic acid was extracted, treated with DNase and RNA sedimenting at about 50S in sucrose gradients was isolated. This method yielded 0.5 to 1.5 µg of genomic RNA per litre of culture. A molecular weight of 4.5 x 10⁶ was determined by gel electrophoresis under fully denaturing conditions.

Keywords: measles virus, RNA purification, mol. wt. determination

Received 9 November 1982; accepted 10 January 1983.