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Repair of Ultraviolet Light-induced DNA Damage in Cholera Bacteriophages

Goutam Das

Department of Biophysics, Indian Institute of Chemical Biology, Calcutta-700 032, India

DNA repair-proficient and -deficient strains of Vibrio cholerae were used to examine host cell reactivation, Weigle reactivation and photoreactivation of u.v.-irradiated cholera bacteriophages. U.v. light-induced DNA damage in phages of different morphological and serological groups could be efficiently photoreactivated. Host cell reactivation of irradiated phages of different groups was different on the same indicator host. Phage ø149 was the most sensitive, and ø138 the most resistant to u.v. irradiation. While ø138 showed appreciable host cell reactivation, this was minimal for ø149. Attempts to demonstrate Weigle reactivation of u.v.-irradiated cholera phages were not successful, although u.v.-induced filamentation of host cells was observed.

Keywords: DNA repair, cholera bacteriophages, Vibrio cholerae

Present address: Department of Radiation Biology and Biophysics, University of Rochester, Rochester, New York 14642, U.S.A.

Received 5 January 1983; accepted 4 April 1983.