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Effects of Actinomycin D on the Infection of Tobacco Protoplasts by Four Viruses

Scottish Crop Research Institute, Invergowrie, Dundee, DD2 5DA, U.K.

The addition of actinomycin D (25 µg/ml) or cordycepin (1 mM) to protoplast cultures immediately after inoculation with particles of tobacco mosaic, tobacco rattle, tobacco ringspot or potato leafroll viruses resulted in a decrease in the proportion of protoplasts becoming infected, as judged by staining with fluorescent antibody to virus particles. A delay of a few hours between the inoculation and the addition of either inhibitor largely or completely eliminated this effect. In contrast, infection was unaffected by the addition of actinomycin D when the protoplasts were inoculated with RNA preparations from tobacco mosaic or tobacco rattle viruses.

Keywords: plant viruses, actinomycin D, cordycepin, tobacco protoplasts

Received 17 January 1983; accepted 11 April 1983.