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Molecular Cloning of the Varicella-Zoster Virus Genome and Derivation of Six Restriction Endonuclease Maps

MRC Virology Unit, Institute of Virology, University of Glasgow, Church Street, Glasgow G11 5JR, U.K.

KpnI and SstI fragments representing 96% of the varicella-zoster virus genome, including the termini, were cloned in plasmid vector pAT153. The clones were used to derive maps of virion DNA for SstI, KpnI, XhoI, PvuII, EcoRI and SalI by molecular hybridization and restriction endonuclease digestion.

Keywords: VZV, cloning, restriction maps

Received 10 March 1983; accepted 13 May 1983.