J Gen Virol Faster Access
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Gen Virol 64 (1983), 1815-1818; DOI 10.1099/0022-1317-64-8-1815
© 1983 Society for General Microbiology
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by King, R. M.
Right arrow Articles by Secher, D. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by King, R. M.
Right arrow Articles by Secher, D. S.
Agricola
Right arrow Articles by King, R. M.
Right arrow Articles by Secher, D. S.

Characterization and Properties of a Modified Human Interferon-{alpha}-Containing an Additional 18 Amino Acids at the N-Terminus

R. M. King, D. C. Burke, F. Northrop1 and D. S. Secher1

Department of Biological Sciences, University of Warwick, Coventry CV4 7AL
and1 Laboratory of Molecular Biology, Medical Research Council Centre, University Medical School, Hills Road, Cambridge CB2 2QH, U.K.

A modified human interferon-{alpha}2 was produced in Escherichia coli cells infected with phage M13 mp7 containing an interferon-{alpha} gene. After purification by immunochromatography with the monoclonal antibody NK2, the N-terminal amino acid sequence was determined. The N-terminal methionine was absent but an additional sequence of 18 amino acids at the N-terminus was retained. The modified interferon-{alpha}2 was indistinguishable from authentic interferon-{alpha}2 in its ability to activate natural killer cells, to slow the growth of Daudi cells, and to confer virus resistance on heterologous cells.

Keywords: interferon (HuIFN-{alpha}), protein sequence, immunochromatography

Received 14 March 1983; accepted 22 March 1983.




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 1983 by the Society for General Microbiology.