HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Davison, A. J. Articles by Wilkie, N. M. Search for Related Content PubMed PubMed Citation Articles by Davison, A. J. Articles by Wilkie, N. M. Agricola Articles by Davison, A. J. Articles by Wilkie, N. M.

Location and Orientation of Homologous Sequences in the Genomes of Five Herpesviruses

N. M. Wilkie

MRC Virology Unit, Institute of Virology, University of Glasgow, Church Street, Glasgow G11 5JR, U.K.

Molecular hybridization experiments were carried out to investigate homologous regions in the genomes of herpes simplex virus types 1 and 2 (HSV-1 and HSV-2), equid herspesvirus 1 (EHV-1), pseudorabies virus (PRV) and varicella-zoster virus (VZV). Virion DNA probes from EHV-1, PRV and VZV hybridized to similar regions of the HSV genome, and the use of cloned DNA probes allowed heterologous genomes to be oriented with respect to homologous regions. The HSV-1 and HSV-2 genomes are colinear, the EHV-1 and VZV genomes are colinear with the I_L or I_SL genome arrangement of HSV, and the PRV genome is essentially colinear with the I_L genome arrangement of HSV except that the region 0.1 to 0.4 fractional genome units appears to be inverted. A detailed analysis of sequences in the HSV-2 and PRV genomes to which the HSV-1 major capsid protein gene hybridized was carried out in order to demonstrate the application of molecular hybridization to the location of genes in heterologous genomes. The lesion in a DNA-positive temperature-sensitive mutant of PRV was mapped within the putative PRV major capsid protein gene. We conclude that the herpesviruses we have studied possess several highly conserved genes, and propose that they are similar in genetic organization despite presumably separate evolutionary histories.

Keywords: herpesviruses, sequence homology, genome analysis

Present address: The Beatson Institute for Cancer Research, Wolfson Laboratory for Molecular Pathology, Garscube Estate, Switchback Road, Glasgow G61 1BD, U.K.

Received 28 February 1983; accepted 18 May 1983.

This article has been cited by other articles:

				Y. Yamagishi, T. Sadaoka, H. Yoshii, P. Somboonthum, T. Imazawa, K. Nagaike, K. Ozono, K. Yamanishi, and Y. Mori Varicella-Zoster Virus Glycoprotein M Homolog Is Glycosylated, Is Expressed on the Viral Envelope, and Functions in Virus Cell-to-Cell Spread J. Virol., January 15, 2008; 82(2): 795 - 804. [Abstract] [Full Text] [PDF]

				B. G. Klupp, C. J. Hengartner, T. C. Mettenleiter, and L. W. Enquist Complete, Annotated Sequence of the Pseudorabies Virus Genome J. Virol., January 1, 2004; 78(1): 424 - 440. [Abstract] [Full Text] [PDF]

				G. W. Kemble, P. Annunziato, O. Lungu, R. E. Winter, T.-A. Cha, S. J. Silverstein, and R. R. Spaete Open Reading Frame S/L of Varicella-Zoster Virus Encodes a Cytoplasmic Protein Expressed in Infected Cells J. Virol., December 1, 2000; 74(23): 11311 - 11321. [Abstract] [Full Text]