J Gen Virol Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Gen Virol 64 (1983), 2079-2085; DOI 10.1099/0022-1317-64-9-2079
© 1983 Society for General Microbiology
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Suh, M.
Right arrow Articles by Frost, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Suh, M.
Right arrow Articles by Frost, E.
Agricola
Right arrow Articles by Suh, M.
Right arrow Articles by Frost, E.

Localization of the Coding Region for a 35000 Dalton Polypeptide on the Genome of Herpes Simplex Virus Type 2

Martha Suh, Christiane Chauvin{dagger}, Mario Filion, Gordon C. Shore{ddagger} and Eric Frost§

Institut du Cancer de Montréal, Centre Hospitalier Notre-Dame, 1560 est, rue Sherbrooke, Montréal, Québec, Canada, H2L 4M1

The cloned BglII N fragment of herpes simplex virus type 2 (HSV-2) DNA has been shown to code for a 35K polypeptide. Subfragments made by cleavage with XhoI, BamHI, SstII and XorII were then cloned and used with RNA extracted from HSV-2-infected cells for mRNA selection and in vitro protein synthesis. We found that the major translation product of such hybrid-selected mRNA has a molecular weight of 35000. By further mapping, DNA coding sequences for this mRNA were located within the region of BglII N, at approximately 0.585 to 0.596 genome map units. DNA sequences complementary to mRNA encoding a 56K polypeptide were located between 0.607 and 0.612 map units.

Keywords: HSV, polypeptides 35K, 56K, hybridization, immunoprecipitation

{dagger} Present address: Laboratoire de Médecine et Chirurgie Expérimentales et Comparées, Cermo Bp 53X - 38041 Grenoble, France.

{ddagger} Present address: Department of Biochemistry, McGill University, Montréal, Canada.

§ Present address: Centre International de Recherches Médicales de Franceville, Franceville, Gabon.

Received 18 March 1983; accepted 18 May 1983.




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 1983 by the Society for General Microbiology.