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Recovery of Herpes Simplex Virus Genetic Information from Human Trigeminal Ganglion Cells following Superinfection with Herpes Simplex Virus Type 2 Temperature-sensitive Mutants

Marcia E. Lewis^1,

¹ Department of Medicine and Neurovirology Research Unit, University of Alberta, Edmonton, Alberta, Canada T6G 2G3
and² MRC Virology Unit, Institute of Virology, Church Street, Glasgow G11 5JR, U.K.

Explant cultures of human trigeminal ganglia were derived from 36 individuals. Those cultures which failed to release herpes simplex virus (HSV) spontaneously were superinfected at various times after establishment in vitro with a range of HSV-2 temperature-sensitive (ts) mutants. Eight cultures from six individuals contained HSV-specific genetic information which could be detected or rescued following superinfection. Restriction enzyme analysis of ts⁺ virus recovered from the ganglia of two individuals following superinfection was identical to that of endogenous HSV-1 spontaneously released from parallel cultures. Retrieval of ts⁺ virus by this technique suggests products of the superinfecting virus activated expression of whole genomes or that spontaneous virus expression occurred unrelated to the act of superinfection.

Keywords: HSV-2 ts mutants, latent HSV, trigeminal ganglia, superinfection

Present address: Department of Therapeutic Radiology, Yale University School of Medicine, New Haven, Connecticut 06510, U.S.A.

Received 2 June 1983; accepted 19 September 1983.

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