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J Gen Virol 65 (1984), 227-231; DOI 10.1099/0022-1317-65-1-227
© 1984 Society for General Microbiology
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Entry of Mouse Hepatitis Virus 3 into Cells

K. Krzystyniak and J. M. Dupuy

Immunology Research Center, Institut Armand-Frappier, Université du Québec, Laval-des-Rapides, Québec, Canada, H7V 1B7

The involvement of lysosomes in infection by mouse hepatitis virus 3 (MHV3) was studied. L cells were infected with MHV3 in the presence of NH4Cl or chloroquine, weak bases which increase the intralysosomal pH and impair lysosomal functions. NH4Cl significantly inhibited virus-induced cytopathic effects and MHV3 replication, but did not prevent the attachment of 3H-labelled virus. No inhibition of MHV3 replication by NH4Cl and chloroquine was observed when lysosomotropic agents were added later than 3 h post-infection, suggesting the direct involvement of lysosomes in release of the viral genome into cytoplasm. These results, together with the lack of antibody-mediated immune lysis of MHV3-infected cells, suggest that MHV3 entered cells by an endocytic pathway (viropexis) followed by internalization into cellular lysosomes.

Keywords: MHV3, endocytosis, lysosomotropic agents

Received 5 June 1983; accepted 12 October 1983.


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