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Rotavirus RNA Segments Sized by Electron Microscopy

MRC Virology Unit and Institute of Virology, University of Glasgow, Church Street, Glasgow G11 5JR, U.K.

The RNA segments of bovine rotavirus (UK Compton strain) were measured by electron microscopy in mixtures containing X174 double-stranded DNA as an internal reference molecule. The numbers of base pairs per segment were calculated and compared with data obtained by other procedures (comparative RNA gel electrophoresis; co-electrophoresis of cDNAs in denaturing gels; sequencing).

Keywords: rotavirus, sizes, RNA segments, EM

Received 26 July 1983; accepted 28 September 1983.