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The Effect of Charge on Infection of Tobacco Protoplasts by Bromoviruses

John Innes Institute, Colney Lane, Norwich NR4 7UH, U.K.

The susceptibility of freshly isolated tobacco protoplasts to infection by brome mosaic virus (BMV) fell rapidly to a low residual level over a period of 8 h in culture. In contrast, susceptibility to infection by cowpea chlorotic mottle virus (CCMV) fell much more slowly over 15 h in culture to a similar residual level. In the absence of polycations, BMV infected many more protoplasts if CCMV were also present in the inoculum even though CCMV did not infect such doubly inoculated protoplasts. Both effects are thought to be a consequence of the different electrical charges of particles of the viruses and support the view that inoculation of protoplasts depends primarily on physical interactions between virus and protoplast.

Keywords: bromoviruses, protoplasts, cowpea chlorotic mottle virus, brome mosaic virus

Received 30 April 1984; accepted 10 July 1984.