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J Gen Virol 65 (1984), 1791-1802; DOI 10.1099/0022-1317-65-10-1791
© 1984 Society for General Microbiology
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Molecular Biological Characterization of a Highly Leukaemogenic Virus Isolated from the Mouse. IV. Viral Proteins

G. A. Dekaban1, J. K. Ball2, W. G. Robey1, L. O. Arthur1 and J. A. McCarter3

1 Frederick Cancer Research Center, Frederick, Maryland 20205, U.S.A.
2 Department of Biochemistry, University of Western Ontario, London, Ontario, Canada N6A 5C1
and3 Department of Microbiology and Biochemistry, University of Victoria, Victoria, British Columbia, Canada V8W 2Y2

The proteins of a highly leukaemogenic murine virus (DMBA-LV) endogenous to the CFW/D mouse have been characterized. This virus does not contain ecotropic, xenotropic or polytropic type C retroviruses capable of replicating in established tissue culture cell lines. Analysis of the viral proteins indicated that proteins characteristic of both type C and type B retroviruses were present. All the proteins characteristic of a competent, mature type B retrovirus, such as the murine mammary tumour virus, were present in the virions and were expressed at high levels in a virus-producing thymic lymphoma cell line. Type C internal structural proteins were detected in the virus and cells producing virus. Type C envelope protein was detected at very low levels in both the virions and the virus-producing tumour cell lines. Chymotryptic polypeptide profiles of this gp70 indicated similarity with the envelope glycoproteins characteristic of xenotropic and recombinant type C retroviruses.

Keywords: leukaemogenic murine virus, proteins, MMTV

Received 2 May 1984; accepted 14 June 1984.




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