HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Hays, E. F. Articles by Silva, R. F. Search for Related Content PubMed PubMed Citation Articles by Hays, E. F. Articles by Silva, R. F. Agricola Articles by Hays, E. F. Articles by Silva, R. F.

Several Classes of Retroviruses Are Produced by an AKR Mouse T Lymphoma Cell Line

The Laboratory of Biomedical and Environmental Sciences, Department of Medicine
and¹ Department of Pathology, School of Medicine, Jonsson Cancer Center, University of California, Los Angeles, California 90024, U.S.A.

Characterization of the viruses produced by the spontaneous T lymphoma cell line SL3 is presented. Using supernatant fluids or direct co-cultivation of cells, the SL3 cell line was found to produce replication-defective viruses in excess of replication-competent viruses. The replication-competent viruses released were predominantly those negative in the XC plaque assay (XC^-); XC⁺ viruses represented a minor population. However, when the SL3-derived viruses were passed in mouse embryo fibroblasts, XC^- viruses were rarely recovered, and XC⁺ viruses were readily isolated. These viruses were all ecotropic and lymphomagenic. Viruses with dual host range and non-oncogenic ecotropic viruses were not isolated from the lymphoma cells. Two replication-defective viruses from SL3 cells were studied. Both could be rescued by non-oncogenic retroviruses and were then lymphomagenic. One defective virus appeared related to XC⁺ viruses. In these studies, the XC⁺ and XC^- viruses appeared to represent two different interference classes using separate cell receptors. Taken together, these experiments show that the SL3 T lymphoma cells replicate a variety of viruses most of which are lymphomagenic. Virus replication and/or virus integration may be the means of maintaining the malignant phenotype of these T lymphoma cells.

Keywords: lymphomagenic retrovirus, AKR mice, replication-defective virus

Received 26 April 1984; accepted 2 August 1984.