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Kilham Rat Virus DNA Replication in Subcellular Fractions

Laboratoire de Biochimie, Institut Curie, Section de Biologie, 26 rue d'Ulm, 75231 Paris Cedex 05, France

A subcellular system actively replicating Kilham rat virus DNA in vitro was developed. Cellular lysates and isolated nuclei from infected cells showed an amplification of replicative forms in vitro. Solubilized replicative complexes, either partly purified or in the form of a crude extract, were able to synthesize replicative forms and single-stranded DNA. DNA polymerase played a major role in Kilham rat virus DNA synthesis in vivo and in vitro. Furthermore, a factor present in the cytosol of infected cells increased the polymerizing activity of viral replicative complexes.

Keywords: Kilham rat virus, DNA replication, DNA polymerase

Received 20 March 1984; accepted 24 July 1984.