HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Harper, D. R. Articles by Samson, A. C. R. Search for Related Content PubMed PubMed Citation Articles by Harper, D. R. Articles by Samson, A. C. R. Agricola Articles by Harper, D. R. Articles by Samson, A. C. R.

Recovery of Host Cell Protein Synthesis during Infection with a Temperature-sensitive Mutant of Newcastle Disease Virus

Department of Genetics, University of Newcastle upon Tyne, Newcastle upon Tyne NE1 7RU, U.K.

When chick embryo fibroblasts infected with high multiplicities of a non-revertible temperature-sensitive (ts) mutant (ts53) of Newcastle disease virus (NDV) at the permissive temperature (34 °C) and incubated to allow the appearance of virus-induced proteins were shifted up to and incubated at the non-permissive temperature (42 °C), analysis of [³H]leucine-labelled proteins by SDS-PAGE revealed a marked increase in cellular protein synthesis and a decline in viral synthesis, with an eventual return to an apparently uninfected state. Mutant ts53 apparently ceased to suppress cellular protein synthesis, and translation of viral proteins was markedly inhibited. This was not observed in ts⁺-infected cells, or in ts53-infected cells maintained at the permissive temperature. Recovery from the infected state was inhibited by actinomycin D at any stage, but was not prevented by cycloheximide present immediately before and during temperature shift-up. Mutant ts53 was shown to be RNA^- at 42 °C, although viral mRNAs synthesized at 34 °C are shown to be present throughout host recovery but in an inactive state.

Keywords: NDV, protein synthesis, ts, IFN

Received 30 May 1984; accepted 10 July 1984.