HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Gray, C. P. Articles by Kaerner, H. C. Search for Related Content PubMed PubMed Citation Articles by Gray, C. P. Articles by Kaerner, H. C. Agricola Articles by Gray, C. P. Articles by Kaerner, H. C.

Sequence of the Putative Origin of Replication in the U_L Region of Herpes Simplex Virus Type 1 ANG DNA

C. P. Gray

Institute for Virus Research, German Cancer Research Center, 6900 Heidelberg, Federal Republic of Germany

Interest has been stimulated concerning the region mapping between 0.38 and 0.42 on the prototype configuration of the herpes simplex virus type 1 (HSV-1) genome due to the high probability of the presence there of a second origin of DNA replication. A 960 bp restriction fragment (HinfI E) of a class II defective HSV-1 ANG DNA has been sequenced using the viral DNA rather than molecularly cloned DNA. This fragment includes the BamHI U/R cleavage site, mapping at approximately 0.4. Part of the sequence derived in this study displays homology with the origins of DNA replication contained in TR_S/IR_S of HSV-1 and HSV-2 DNA. The homologous region comprising 76 bp occurs as two copies, each of which contains two palindromically arranged copies of an 8 bp sequence identical to the ‘consensus’ sequence reported to be part of the origin of DNA replication at the terminus of the mammalian adenoviruses. It can be deduced from a comparison of this structure to the TR_S/IR_S origin of HSV-1 and HSV-2 that there are two origins of replication in the U_L region of HSV-1 ANG DNA. Assuming that the orientation of the consensus sequence is relevant to the direction of DNA replication, one can conclude that the U_L origin(s) of HSV-1 ANG is (are) bidirectional. It has not yet been possible to clone DNA fragments molecularly which include the region spanning the U_L origin(s) of HSV-1 DNA.

Keywords: HSV-1, U_L, origin of replication

Present address: EMBL, Meyerhofstrasse 1, 6900 Heidelberg, F.R.G.

Received 5 June 1984; accepted 29 August 1984.

This article has been cited by other articles:

				L. Perelygina, L. Zhu, H. Zurkuhlen, R. Mills, M. Borodovsky, and J. K. Hilliard Complete Sequence and Comparative Analysis of the Genome of Herpes B Virus (Cercopithecine Herpesvirus 1) from a Rhesus Monkey J. Virol., June 1, 2003; 77(11): 6167 - 6177. [Abstract] [Full Text] [PDF]