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J Gen Virol 65 (1984), 2173-2181; DOI 10.1099/0022-1317-65-12-2173
© 1984 Society for General Microbiology
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Comparison of Dengue Viruses and Some Other Flaviviruses by cDNA-RNA Hybridization Analysis and Detection of a Close Relationship between Dengue Virus Serotype 2 and Edge Hill Virus

J. Blok, E. A. Henchal1 and B. M. Gorman

Queensland Institute of Medical Research, Bramston Terrace, Herston, Brisbane, Queensland 4006, Australia
and1 Walter Reed Army Institute of Research, Walter Reed Army Medical Center, Washington, D.C. 20307, U.S.A.

Variable amounts of cDNA were synthesized in vitro from RNA extracted from several flaviviruses, including the four prototype dengue (DEN) virus serotypes. The synthesis was carried out using an oligo(dT) primer, suggesting the presence of a short poly(A) region at or near the 3' end of some flavivirus genomes. The DEN-1 and DEN-2 prototype strains produced the largest amount of cDNA and were therefore used to investigate further the relatedness of flavivirus genomes by cDNA-RNA hybridization. The flaviviruses studied are related to each other to some extent since the hybrids formed exhibited about 30% S1 nuclease resistance, but a closer relationship was detected between dengue viruses of serotype 1 and 4 and between dengue virus serotype 2 and Edge Hill virus. A monoclonal antibody to the envelope protein (V3) of dengue viruses reacted with Edge Hill virus, confirming the genetic relationship between the viruses.

Keywords: flaviviruses, dengue virus RNA, cDNA-RNA hybridization

Received 27 March 1984; accepted 9 August 1984.




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