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A Comparison of the Neutralizing Properties of Monoclonal and Polyclonal Antibodies to Human Interferon Alpha

Division of Viral Products, National Institute for Biological Standards and Control, Holly Hill, Hampstead, London NW3 6RB, U.K.

Specific polyclonal antisera to human interferon-1 (HuIFN-1), human interferon-2 (HuIFN-2) and human lymphoblastoid interferon (HuIFN-Ly Namalwa) have been raised in rabbits and sheep. The antisera raised against HuIFN-1 and HuIFN-2 strongly neutralized the antiviral activity of their homologous IFN- subtypes, but were less active against the heterologous IFN- subtypes and preparations containing mixtures of IFN- subtypes, e.g. human leukocyte interferon (HuIFN-Le). Antisera raised against HuIFN-Ly Namalwa strongly neutralized the antiviral activity of all IFN--containing preparations and showed weak cross-reactivity with human interferon- (HuIFN-). Neither anti-HuIFN-1 nor anti-HuIFN-2 could be demonstrated to neutralize the antiviral activity of HuIFN-. A number of monoclonal antibodies to HuIFN-2 have been prepared and these were found to neutralize HuIFN-2 antiviral activity to varying degrees, but not to neutralize the heterologous subtype HuIFN-1, preparations containing mixtures of IFN- subtypes or HuIFN-.

Keywords: IFN (HuIFN-), monoclonal and polyclonal antibodies, neutralization

Received 16 May 1984; accepted 29 August 1984.