HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Mockett, A. P. A. Articles by Brown, T. D. K. Search for Related Content PubMed PubMed Citation Articles by Mockett, A. P. A. Articles by Brown, T. D. K. Agricola Articles by Mockett, A. P. A. Articles by Brown, T. D. K.

Monoclonal Antibodies to the S1 Spike and Membrane Proteins of Avian Infectious Bronchitis Coronavirus Strain Massachusetts M41

Department of Microbiology, Houghton Poultry Research Station, Houghton, Huntingdon, Cambs. PE17 2DA, U.K.

We have established four murine hybridoma cell lines which secrete specific antibody to avian infectious bronchitis virus (IBV) strain Massachusetts M41. Two monoclonal antibodies reacted with the spike protein and two reacted with the membrane protein. The specificity of the monoclonal antibodies for the external structural proteins was detected by immunoprecipitations using radiolabelled virus. The reactions of the monoclonal antibodies showed that one (S1) of the two glycopolypeptides associated with the spike protein has a strain-specific region involved in neutralization and haemagglutination, and the membrane protein has antigenic determinants which are present on the three strains of IBV tested (M41, Beaudette and D41).

Keywords: coronavirus, IBV, monoclonal antibodies

Received 27 April 1984; accepted 10 September 1984.

This article has been cited by other articles:

				C. Winter, C. Schwegmann-Wessels, D. Cavanagh, U. Neumann, and G. Herrler Sialic acid is a receptor determinant for infection of cells by avian Infectious bronchitis virus. J. Gen. Virol., May 1, 2006; 87(Pt 5): 1209 - 1216. [Abstract] [Full Text] [PDF]

				O. Krauss, R. Hollinshead, M. Hollinshead, and G. L. Smith An investigation of incorporation of cellular antigens into vaccinia virus particles J. Gen. Virol., October 1, 2002; 83(10): 2347 - 2359. [Abstract] [Full Text] [PDF]