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Friend Erythroleukaemia Cell Mutants Defective in Viral Gene Expression

A. Iwamoto

Atsuko Suzuki

Y. Kitamura

H. Yoshikura

Department of Genetics, Institute of Medical Science, University of Tokyo, Tokyo 108, Japan

Cellular mutants defective in the expression of viral polypeptides were isolated from the Friend erythroleukaemia cell line 745a by immunoselection with anti-ecotropic murine leukaemia virus serum in the presence of complement. The mode of appearance of the antiserum-resistant cells showed an interesting pattern which is discussed in the text. One of the mutants obtained contained no detectable gp70 or p15(E) while retaining gPr90^env; defects appeared to reside in the processing of the gPr90^env to gp70 and p15(E). In another type of mutant, gPr90^env was not detected. All these mutants retained spleen focus-forming virus (SFFV)-specific gp55 and gag precursor Pr68^gag. The mutants superinfected with the helper virus produced the helper and SFFV also, indicating that these mutations did not affect the replication of the exogenously infecting virus.

Keywords: Friend cell mutant, immunoselection, MuLV antiserum

Present address: Department of Bacteriology, Faculty of Medicine, University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo, Japan.

Present address: Department of Biochemistry, Cancer Institute, Toshima-ku, Tokyo, Japan.

Received 7 February 1983; accepted 22 September 1983.