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A Physical Map for the Heliothis zea SNPV Genome

J. D. Knell^,,

M. D. Summers

Department of Entomology, Texas A & M University and the Texas Agricultural Experiment Station, College Station, Texas 77843, U.S.A.

Restriction sites on the Heliothis zea nuclear polyhedrosis virus (HzSNPV) genome were mapped for the endonucleases BamHI, EcoRI, HindIII, SstI, XhoI and KpnI. XhoI-F was used as the zero-point of the physical map because it hybridized to the HindIII-V fragment which codes for the 3' end of the polyhedrin gene of the Autographa californica nuclear polyhedrosis virus (AcMNPV). The DNA homology between eight AcMNPV cloned fragments and HzSNPV DNA was mapped using Southern hybridization and the locations of HzSNPV DNA sequences sharing homology with AcMNPV gene sequences are discussed.

Keywords: HzSNPV, baculovirus, DNA homology, physical map

Present address: Section of Cardiology, Baylor College of Medicine, The Methodist Hospital, Houston, Texas 77030, U.S.A.

Photographic copies of radiographs and other materials used in this study are available from either author.

Received 31 May 1983; accepted 13 October 1983.