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The Junctions between the Repetitive and the Short Unique Sequences of the Herpes Simplex Virus Genome Are Determined by the Polypeptide-coding Regions of Two Spliced Immediate-early mRNAs

Institute of Virology, University of Glasgow, Church Street, Glasgow G11 5JR, U.K.

Immediate-early (IE) mRNAs-4 and -5 of herpes simplex virus type 2 (HSV-2) are transcribed from the IR_S/TR_S genome regions towards the U_S region. Each of these spliced mRNAs has an untranslated leader sequence of 249 bases and a single intron of approximately 540 bases which are contained entirely within TR_S/IR_S sequences. The DNA sequence of the intron largely comprises tandem reiterations of three distinct short sequences. Upstream of the common 5' mRNA termini the DNA sequence contains regions of homology with the equivalent region of HSV-1. Comparison of the polypeptides encoded by these HSV-2 mRNAs with those of HSV-1 shows blocks of conserved amino acids. The locations of the first initiator ATG triplets of these two HSV-2 mRNAs suggest that the IR_S/TR_S regions of HSV expand, by gene conversion or by equal though non-homologous crossover, to an extent determined by the functions of the DNA sequences which are duplicated or deleted as a result of the crossover. This mechanism for expansion of repeats may apply to other herpesviruses which have a genome structure similar to that of HSV.

Keywords: HSV-1 and -2, spliced IE mRNAs, repetitive sequences, gene conversion

Received 27 October 1983; accepted 6 December 1983.

This article has been cited by other articles:

				A. Dolan, F. E. Jamieson, C. Cunningham, B. C. Barnett, and D. J. McGeoch The Genome Sequence of Herpes Simplex Virus Type 2 J. Virol., March 1, 1998; 72(3): 2010 - 2021. [Abstract] [Full Text] [PDF]