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J Gen Virol 65 (1984), 617-627; DOI 10.1099/0022-1317-65-3-617
© 1984 Society for General Microbiology
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Isolation and Biological Characterization of Mouse LM Fibroblast Variants Resistant to the Cytotoxicity of Polyriboinosinic·Polyribocytidylic Acid after Interferon Treatment

Thérèse Faure1, Pierre G. Milhaud2, Hélène Fruteau de Laclos1, Nadir Mechti1, Michèle Silhol1, Georges Huez3 and Bernard Lebleu1

1 Laboratoire de Biochimie des Protéines et
2 Laboratoire de Biologie Moléculaire, ERA CNRS 482, Université de Montpellier II, Place E. Bataillon, 34060 Montpellier Cédex, France
and3 Laboratoire de Chimie Biologique, Université Libre de Bruxelles, Belgium

Mouse LM fibroblasts growing continuously in the absence of serum have an increased sensitivity to the cytotoxicity of poly(rI)·poly(rC) after interferon (IFN) exposure. This has allowed the isolation by an enrichment procedure of several independent and stable variant clones (IFN + I·C)R which are resistant to such a treatment. One of the resistant variants has been more extensively characterized as far as IFN action and IFN production are concerned. It behaves identically to the wild-type parent except for the spontaneous release of low amounts of IFN. The target of the mutation probably resides in a late step in the development of the cytotoxic response as revealed by microinjection techniques. The (IFN + I·C)R variants characterized here thus appear different from mutants in the IFN system isolated so far.

Keywords: LM fibroblasts, IFN, poly(rI)·poly(rC)

Received 28 October 1983; accepted 15 November 1983.




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Copyright © 1984 by the Society for General Microbiology.