HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Stecher, A. Articles by Schügerl, K. Search for Related Content PubMed PubMed Citation Articles by Stecher, A. Articles by Schügerl, K. Agricola Articles by Stecher, A. Articles by Schügerl, K.

Interferon- Yields from Sendai Virus-induced Human Leukocyte Cultures Are Enhanced by Lowering the Incubation Temperature

Institut für Technische Chemie der Universität Hannover, Callinstrasse 3, D-3000 Hannover 1, Federal Republic of Germany
and¹ DRK-Blutspendedienst Niedersachsen, Eldagsener Strasse 38, D-3257 Springe 1, Federal Republic of Germany

Increased yields of Sendai virus-induced human leukocyte interferon (IFN-) were obtained by lowering the incubation temperature from the initial 37°C within 1 to 6 h after the addition of virus. Maximal stimulation was found when this was done 2 to 4 h after induction and when the lowered temperature was between 29 and 32°C. Compared to cultures kept at 37°C throughout, the resulting IFN- yields were enhanced by 2.5- to 3-fold. This was mainly due to prolonged synthesis of IFN-, which could be observed up to 20 h after induction, whereas in cultures kept at 37°C the production of IFN- had practically ceased approx. 10 h after the addition of virus.

Keywords: IFN, leukocyte culture, temperature, yields

Received 26 August 1983; accepted 21 November 1983.