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J Gen Virol 65 (1984), 799-802; DOI 10.1099/0022-1317-65-4-799
© 1984 Society for General Microbiology
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Characterization of Three Highly Purified Influenza Virus Strains by Electron Microscopy

R. W. H. Ruigrok, P. J. Andree, R. A. M. Hooft Van Huysduynen and J. E. Mellema

Department of Biochemistry, State University of Leiden, Wassenaarseweg 64, 2333 AL Leiden, The Netherlands

Mass determinations on highly purified influenza virus preparations were performed using the technique of scanning transmission electron microscopy. The masses of the three strains, X49, B/Singapore/222/79 and B/Hong Kong/8/73 were determined. The average value was 174 x 106 daltons with only small differences between the three strains. The mass of virus particles after removal of the protruding spike proteins, haemagglutinin and neuraminidase by bromelain treatment was determined to be 86 x 106 daltons. From the mass difference and the known molecular weight of the spike proteins the number of spikes was estimated to lie in the range 400 to 500.

Keywords: influenza virus, mass determination, composition, STEM

Received 4 October 1983; accepted 26 January 1984.


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A. Mittal, T. Shangguan, and J. Bentz
Measuring pKa of Activation and pKi of Inactivation for Influenza Hemagglutinin from Kinetics of Membrane Fusion of Virions and of HA Expressing Cells
Biophys. J., November 1, 2002; 83(5): 2652 - 2666.
[Abstract] [Full Text] [PDF]


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