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A Cloning Strategy for the Bacteriophage T4 vs Gene

Section on Microbiology, Division of Biology, Kansas State University, Manhattan, Kansas 66506, U.S.A.

The product of the bacteriophage T4 vs gene, the peptide, has been shown to thermally stabilize temperature-sensitive valyl-tRNA synthetase (EC 6.1.1.9 [EC] ). To clone the bacteriophage T4 vs gene, recombinant pBR322-T4 DNA molecules were used to transform Escherichia coli CP 790302 (valS^ts). Transformants that grew at 43.5 °C on selective medium exhibited some properties indicative of a phage-modified valyl-tRNA synthetase. The data are consistent with successful cloning of the vs gene and atypical modification of the valyl-tRNA synthetase in CP 790302.

Keywords: phage T4, cloning, valyl-tRNA synthetase

Received 29 September 1983; accepted 12 January 1984.