HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Shirako, Y. Articles by Brakke, M. K. Search for Related Content PubMed Articles by Shirako, Y. Articles by Brakke, M. K. Agricola Articles by Shirako, Y. Articles by Brakke, M. K.

Spontaneous Deletion Mutation of Soil-borne Wheat Mosaic Virus RNA II

Yukio Shirako

Agricultural Research Service, U.S. Department of Agriculture and Department of Plant Pathology, Nebraska Agricultural Experiment Station, University of Nebraska, Lincoln, Nebraska 68583, U.S.A.

The wild-type (WT) isolate of soil-borne wheat mosaic virus has two species of rod-shaped virions 281 nm and 138 nm in length, which are designated as virions 1.0L and 0.5L, respectively. We reported previously that virions shorter than 0.5L arose in assay plants inoculated with separated and recombined 1.0L RNA and 0.5L RNA and suggested that these short virions were caused by deletion mutation of 0.5L RNA. We now report that these short virions arose after a period of several months in wheat plants that had been inoculated manually with unpurified WT isolate, and also when plants infected naturally in fields infested by the fungal vector, Polymyxa graminis, were grown at 17 °C. The sizes and relative proportions of virions shorter than 0.5L varied both from plant to plant and in the same plant sampled at different times. This indicates that the virions shorter than 0.5L arose by continued and spontaneous deletion mutation of 0.5L RNA.

Keywords: SBWMV, deletion mutant, Polymyxa graminis

Present address: Faculty of Agriculture, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Sendai 980, Japan.

Received 25 October 1983; accepted 24 January 1984.