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Monoclonal Antibodies to Hepatitis Be Antigen (HBeAg) Derived from Hepatitis B Core Antigen (HBcAg): Their Use in Characterization and Detection of HBeAg

Department of Virology (Microbiology), Middlesex Hospital Medical School, Riding House Street, London W1P 7LD, U.K.

A panel of mouse hybridomas secreting monoclonal antibody to serum hepatitis Be antigen (HBeAg) was produced from mice immunized with denatured hepatitis B core antigen (HBcAg). This panel could be divided broadly into two groups. Within each group, the monoclonal antibodies recognized a single antigenic site, designated either e- or e-, and generally exhibited a high degree of cross-inhibition. In contrast, between the two groups of antibodies there was little or no cross-inhibition. The antigens of serum HBeAg and denatured HBcAg appeared to be very similar. Both behaved as molecules carrying only a single e- or e- site, in spite of native serum HBeAg having an apparent molecular weight of 300000. It is inferred that e- and e- sites may be involved in the polymerization of HBeAg into HBcAg and that during this process mutual masking of antigenic sites may occur.

Keywords: HBeAg to HBcAg conversion, MAb, HBV

Received 30 November 1983; accepted 20 February 1984.

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