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Properties of Sindbis Virus Variants from Infected Culex tarsalis Mosquitoes

B. T. Eaton^2,

¹ Department of Biology
and² Department of Microbiology, Queen's University, Kingston, Ontario K7L 3N6, Canada

Plaque-purified Sindbis virus was passed three times in Culex tarsalis mosquitoes and progeny viruses were isolated by plaque purification on a cloned line of Aedes albopictus cells. Nine of ten clones examined differed from wild-type (wt) virus with respect to their plaque morphology characteristics in chick embryo fibroblast (CEF) and/or A. albopictus cells. Seven clones were temperature-sensitive and failed to replicate or synthesize viral RNA in CEF cells at 41 °C. At 35 °C in CEF cells the majority of isolates synthesized less viral RNA than wt virus. In contrast, all cloned isolates synthesized viral RNA more rapidly than wild-type virus in A. albopictus cells.

Keywords: Sindbis virus, mosquitoes, temperature sensitivity

Present address: Australian National Animal Health Laboratory, C.S.I.R.O., P.O. Bag 24, Geelong, Victoria, 3220, Australia.

Received 6 January 1984; accepted 30 January 1984.