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Propagation of Rotaviruses in the Presence of Chicken Serum

E. Michael Twist^1,

¹ Division of Infectious Disease, The Children's Hospital of Philadelphia, Philadelphia, Pennsylvania
and² Departments of Medicine and Microbiology, University of Pennsylvania, 533 Johnson Pavilion G2, Philadelphia, Pennsylvania 19104, U.S.A.

The ability of mammalian rotaviruses to replicate in BSC-1 and CV-1 cell cultures was facilitated by the presence of 4% chicken serum. Viral plaques tended to be larger and appear more quickly than in cultures without added chicken serum. It is proposed that chicken serum facilitates plaquing of rotavirus due to its lack of trypsin inhibitors.

Keywords: rotavirus, trypsin, chicken serum, foetal bovine serum

Present address: International Diagnostic Technology, 2986 Oakmead Village Court, Santa Clara, California 95051, U.S.A.

Received 20 December 1983; accepted 21 March 1984.