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Plaque Titration and Inhibition Tests for Bovine Parvovirus

P. J. K. Durham

Department of Tropical Veterinary Science, James Cook University, Townsville, Queensland 4811, Australia

Bovine parvovirus readily produced plaques when inoculated into 60% confluent, actively growing bovine embryonic lung cells. Incorporation of DEAE-dextran, MgCl₂ and DMSO in the agarose overlay medium was found to improve plaque production, especially with the latter chemical. In contrast, protamine sulphate inhibited plaque development. It was found that plaque titration and plaque inhibition tests could be conveniently carried out in 24-well cell culture plates, using an agarose overlay containing DMSO, DEAE-dextran and foetal calf serum. The procedures were highly sensitive, when compared with other established techniques.

Keywords: bovine parvovirus, plaque titration, plaque inhibition

Present address: Animal Health Reference Laboratory, Wallaceville Animal Research Centre, Private Bag, Upper Hutt, New Zealand.

Received 3 February 1984; accepted 2 May 1984.