HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Gambke, C. Articles by Alkan, S. S. Search for Related Content PubMed PubMed Citation Articles by Gambke, C. Articles by Alkan, S. S. Agricola Articles by Gambke, C. Articles by Alkan, S. S.

Monoclonal Antibodies Recognizing Structural Components of Murine Retroviruses Including an FMR Antigen on Protein p12

¹ Friedrich Miescher-Institut, P.O. Box 2543, CH-4002 Basel, Switzerland
² Research Department, Pharmaceuticals Division, Ciba-Geigy Ltd., CH-4002 Basel, Switzerland
and³ Institute for Immunobiology, D-7800 Freiburg, Federal Republic of Germany

Monoclonal antibodies were prepared from mice and rats immunized with Friend leukaemia virus and BALB/c xenotropic virus. By immunoprecipitation of ¹²⁵I-labelled and [³⁵S]methionine-labelled viruses and by protein blotting, ten antibodies were found to react with the viral components p12, p15, p30, gp70 and p15E/p12E. A dot-immunobinding assay was found to be a reliable method to type the antibody reactivity with different murine leukaemia viruses (MuLVs). When tested on a panel of ecotropic and xenotropic MuLVs the antibodies revealed the following antigenic specificities: ecotrop-specific on p15E/p12E; xenotrop-specific on p15E; group-specific on p30 and p15E; FM-specific on gp70; FR-specific on gp70 and p15. Of particular interest is a cytotoxic antibody recognizing an FMR determinant localized on p12.

Keywords: monoclonal antibodies, retrovirus proteins, FMR antigen

Received 24 January 1984; accepted 16 May 1984.