HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Hibi, T. Articles by Saito, Y. Search for Related Content PubMed Articles by Hibi, T. Articles by Saito, Y. Agricola Articles by Hibi, T. Articles by Saito, Y.

Double-stranded RNA of Rice Gall Dwarf Virus

Laboratory of Applied Microbiology, Department of Applied Physiology, National Institute of Agrobiological Resources, Tsukuba Science City, Yatabe, Ibaraki 305
¹ Laboratory of Virus Disease Control, Department of Plant Protection, National Agriculture Research Center, Tsukuba Science City, Yatabe, Ibaraki 305
and² Division of Microbiology, Department of Environmental Biology, National Institute of Agro-Environmental Sciences, Tsukuba Science City, Yatabe, Ibaraki 305, Japan

Nucleic acid extracted from purified particles of rice gall dwarf virus (RGDV) was identified as double-stranded RNA because it (i) was susceptible to RNase A in 0.1 x standard saline citrate (SSC) but not in 1 x SSC, (ii) was resistant to nucleae S1, (iii) showed no hyperchromicity in u.v. absorption after treatment with formaldehyde at 37 °C, (iv) showed a sharp thermal transition in u.v. absorption in 0.01 x SSC and (v) had a buoyant density of 1.596 g/ml in Cs₂SO₄. Electrophoresis in polyacrylamide slab gel revealed that RGDV RNA was composed of 12 segments with a total mol. wt. of about 16.9 x 10⁶. Co-electrophoresis of RNA from RGDV and rice dwarf virus demonstrated that the electrophoretic mobilities of the seven larger segments from the two viruses were similar but that the five smaller segments differed in this respect. These results confirm that RGDV is a new virus and a third member of the genus Phytoreovirus.

Keywords: RGDV, dsRNA, Phytoreovirus, segmented genome

Received 13 March 1984; accepted 23 May 1984.