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J Gen Virol 65 (1984), 1607-1610; DOI 10.1099/0022-1317-65-9-1607
© 1984 Society for General Microbiology
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Characterization of the Genome of a Rod-shaped Virus Infecting Spiroplasma citri

Matthew J. Dickinson and Rod Townsend

Department of Virus Research, John Innes Institute, Colney Lane, Norwich NR4 7UH, U.K.

The genome of a rod-shaped virus, aa, isolated from Spiroplasma citri, has been examined. Electron microscopy and single strand-specific S1 nuclease digestion showed that the genome is a single-stranded circular molecule of 8.5 kilobases. Two replicative forms of DNA have been isolated, a supercoiled double-stranded circular molecule and a nicked form derived from it. A physical map of the replicative forms has been constructed.

Keywords: spiroplasma virus, Spiroplasma citri, replicative forms

Received 12 March 1984; accepted 29 May 1984.




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