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J Gen Virol 66 (1985), 2589-2596; DOI 10.1099/0022-1317-66-12-2589
© 1985 Society for General Microbiology
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Translation of Tobacco Ringspot Virus RNA in Reticulocyte Lysate: Proteolytic Processing of the Primary Translation Products

Stephen A. Jobling{dagger} and Roger K. Wood

Department of Microbiology, University of Birmingham, Birmingham B15 2TT, U.K.

Tobacco ringspot virus (TobRV) RNA was translated efficiently in rabbit reticulocyte lysate and directed the synthesis of two principal polypeptides, Mr 207 x 103 (207K) and 116K, corresponding to the translation products of RNA-1 and RNA-2 respectively. In addition, a 112K RNA-2-encoded polypeptide was sometimes detected. The 116K polypeptide was immunoprecipitated with anti-TobRV serum, suggesting that it was a precursor to coat protein. When translations were performed in the presence of dithiothreitol, the 207K polyprotein was apparently cleaved to yield 37K and 180K polypeptides, with additional processing into 65K and 128K polypeptides. Cleavage of the RNA-2-encoded polyprotein also occurred, although to a much lesser extent than that of the 207K polyprotein; polypeptides of 88K and 54K, both immunoprecipitated with antiviral serum, were identified as RNA-2-encoded cleavage products.

Keywords: TobRV, translation in vitro, proteolytic processing

{dagger} Present address: Division of Health Sciences and Technology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, U.S.A.

Received 22 April 1985; accepted 14 August 1985.




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Copyright © 1985 by the Society for General Microbiology.