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A Physical Map of the Oryctes Baculovirus Genome

¹ DSIR Entomology Division, Department of Scientific and Industrial Research, Private Bag, Auckland, New Zealand
² Department of Cell Biology, Auckland University, Private Bag, Auckland, New Zealand
and³ Microbiology Department, Queen's University, Kingston, Ontario, Canada

The restriction endonucleases, BamHI, EcoRI, HindIII and PstI, cleave Oryctes baculovirus (strain PV505) DNA into 21, 43, 23 and seven fragments respectively. A large number of these fragments were cloned into the bacterial plasmids pUC8 and pBR328. These clones encompass 96% of the genome. The restriction sites for the four endonucleases were mapped using double and partial digestions of cloned fragments as well as hybridization of labelled fragments to Southern transfers of cleaved DNA. When HindIII fragment D and BamHI fragment D were hybridized to Southern transfers six regions containing reiterated sequences were found. The physical map for Oryctes baculovirus could not be orientated with respect to other published baculovirus maps because the BamHI fragment F of Autographa californica nuclear polyhedrosis virus which contains conserved polyhedrin gene sequences common to occluded baculoviruses did not bind to Oryctes baculovirus DNA.

Keywords: Oryctes rhinoceros, baculovirus, restriction endonuclease map, reiterated sequences

Received 4 June 1985; accepted 28 August 1985.