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J Gen Virol 66 (1985), 2787-2792; DOI 10.1099/0022-1317-66-12-2787
© 1985 Society for General Microbiology
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Restriction Endonuclease Analysis of Bovine Herpesvirus 1 DNA and Nucleic Acid Homology between Isolates

Bruce S. Seal1, Stephen C. St. Jeor1 and Robert E. Lee Taylor2

1 Departments of Microbiology and Biochemistry, University of Nevada School of Medicine, Reno, Nevada 89557
and2 Agriculture Experiment Station, University of Nevada School of Veterinary Medicine, Reno, Nevada 89502, U.S.A.

Isolates of bovine herpesvirus 1 (BHV-1) are associated with a variety of clinical manifestations. To determine if a single form of BHV-1 was responsible for the different virus-associated diseases or whether subpopulations of various isolates produced different clinical symptoms, studies were initiated to examine the DNA restriction enzyme patterns and nucleic acid homology between virus isolates from respiratory infections and other clinical syndromes. Differences between the genomes of several virus isolates were detected using DNA restriction enzyme analyses. However, nucleic acid hybridization studies of the virus DNAs using filter and liquid hybridization indicated at least a 95% genetic homology between the virus isolates from different types of infections. Additionally, these studies demonstrated that the DNA of BHV-1 had an average molecular weight of 84 x 106.

Keywords: BHV-1, field isolates, laboratory isolates, restriction enzyme analysis

Received 22 May 1985; accepted 20 August 1985.




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Copyright © 1985 by the Society for General Microbiology.