HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Cunningham, A. L. Articles by Merigan, T. C. Search for Related Content PubMed PubMed Citation Articles by Cunningham, A. L. Articles by Merigan, T. C. Agricola Articles by Cunningham, A. L. Articles by Merigan, T. C.

Interferon Gamma Production by Herpes Simplex Virus Antigen-specific T Cell Clones from Patients with Recurrent Herpes Labialis

Anthony L. Cunningham

Patricia A. Nelson

Divisions of Infectious Diseases and Immunology, Department of Medicine, Stanford University Medical Center, Stanford, California 94305, U.S.A.

Nineteen herpes simplex virus (HSV) antigen-specific human T lymphocyte clones were established from three volunteers with recent recurrent herpes labialis. All produced interferon gamma (IFN-) at titres of 200 to 700 units/ml when cultured in vitro with HSV antigen and irradiated peripheral blood mononuclear cells (PBMC) as filler cells. All 10 of those clones whose phenotype was determined were Leu 4⁺, Leu 2^-, Leu 3⁺. Interleukin 2 alone failed to induce IFN- in titres greater than 10 units/ml from these clones cultured at 10⁴/0.2 ml/well. However, the effect of different accessory or filler cells on IFN- production by clones was quite marked. For example, high titres were produced when irradiated PBMC or plastic-adherent cells (predominantly monocytes) were added and low titres when macrophages and irradiated Epstein-Barr virus-transformed B (EBV-B) cells were added. When tested for HSV antigen-stimulated IFN production alone, the irradiated PBMC and adherent cells produced low titres, but no detectable interferon was produced by the others. However, with higher concentrations of EBV-B cells, low concentrations of IFN- were occasionally produced. Irradiation strikingly reduced IFN- production by PBMC. The IFN- and - produced by accessory cells may contribute to total IFN production by priming the production by cloned cells, and acting in synergy with IFN- produced by the cloned cells. Alternatively, the effect may be due to the presence of permissive concentrations of other lymphokines such as the interleukins. Interferon production by cloned T lymphocytes in the presence of non-producing macrophages was maximal within 24 h, much faster than with a similar polyclonal system, although attaining lower titres. EBV-B cells from only one of three patients supported antigen-specific lymphocyte activation. Almost all cells of the three cell lines expressed DR antigens, while DS/DC antigens were also expressed on nearly all cells of the antigen-presenting line and, at lower densities, on two-thirds of the cells of the other two lines.

Keywords: HSV, interferon (HuIFN-), herpes labialis, T cells

Present address: Department of Virology, Institute of Clinical Pathology and Medical Research, Westmead Centre, Westmead, N.S.W. 2145, Australia.

Present address: Carcinex Inc., Burlingame, California, U.S.A.

Received 18 June 1984; accepted 16 October 1984.

This article has been cited by other articles:

				M. A. Fernandez, F. K. Puttur, Y. M. Wang, W. Howden, S. I. Alexander, and C. A. Jones T Regulatory Cells Contribute to the Attenuated Primary CD8+ and CD4+ T Cell Responses to Herpes Simplex Virus Type 2 in Neonatal Mice J. Immunol., February 1, 2008; 180(3): 1556 - 1564. [Abstract] [Full Text] [PDF]

				S. Barcy and L. Corey Herpes Simplex Inhibits the Capacity of Lymphoblastoid B Cell Lines to Stimulate CD4+ T Cells J. Immunol., May 15, 2001; 166(10): 6242 - 6249. [Abstract] [Full Text] [PDF]