J Gen Virol Try Microbiology Online
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Gen Virol 66 (1985), 303-307; DOI 10.1099/0022-1317-66-2-303
© 1985 Society for General Microbiology
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rombaut, B.
Right arrow Articles by Boeyé, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rombaut, B.
Right arrow Articles by Boeyé, A.
Agricola
Right arrow Articles by Rombaut, B.
Right arrow Articles by Boeyé, A.

Stabilization by Host Cell Components and Mg2+ of the Neutralization Epitopes of Poliovirus

Bart Rombaut, Raf Vrijsen and Albert Boeyé

Department of Microbiology and Hygiene, Vrije Universiteit Brussel, Laarbeeklaan 103, B-1090 Brussels, Belgium

When type 1 poliovirions were extracted from infected HeLa cells and heated at 56 °C in the presence of 1 mM-Mg2+, RNA-free, pluri-antigenic particles were formed. These particles possessed neutralization epitopes as well as epitopes associated with H antigen and with the isolated capsid protein VP3. The heating caused the formation of exclusively H antigenic particles when the Mg2+ concentration was 10-4 M or lower, or when the virus was further purified in order to remove remaining cellular components. The ability of purified virus to form pluri-antigenic particles upon heating could be restored by incubation at 37 °C with infected cell extract.

Keywords: poliovirus, heat inactivation, pluri-antigenic particles

Received 11 June 1984; accepted 16 October 1984.


This article has been cited by other articles:


Home page
 J. Virol.Home page
W. Chehadeh, P.-E. Lobert, P. Sauter, A. Goffard, B. Lucas, J. Weill, M.-C. Vantyghem, G. Alm, P. Pigny, and D. Hober
Viral Protein VP4 Is a Target of Human Antibodies Enhancing Coxsackievirus B4- and B3-Induced Synthesis of Alpha Interferon
J. Virol., November 15, 2005; 79(22): 13882 - 13891.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 1985 by the Society for General Microbiology.