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A Viroid-like RNA Isolated from Grapevine Has High Sequence Homology with Hop Stunt Viroid

Department of Botany, Faculty of Agriculture, Hokkaido University, Sapporo 060
¹ Department of Biophysics and Biochemistry, Faculty of Science, University of Tokyo, Tokyo 113
and² Department of Agricultural Chemistry, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Fuchu 183, Japan

Viroid-like RNA was isolated from some samples of grapevine leaves (Vitis vinifera L.). When inoculated to cucumber, this RNA induced symptoms that were identical to those induced by hop stunt viroid (HSV). It had a molecular size similar to that of HSV, and hybridized with a ³²P-labelled DNA probe for the cucumber isolate of HSV (HSV-c, pCP-55). This hybridization suggests high (> 55%) sequence homology with HSV. The HSV-related sequence was detected not only in the infected cucumber plants but also directly in RNA extracted from grapevine plants. The result indicates that viroid-like RNA, closely related to HSV, is present in grapevines in Japan.

Keywords: viroid, grapevine, hop stunt viroid

Received 15 June 1984; accepted 8 October 1984.