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Isolation of a Mutant of Cowpea Mosaic Virus which is Unable to Grow in Cowpeas

David Evans

John Innes Institute, Colney Lane, Norwich NR4 7UH, U.K.

Following nitrous acid mutagenesis of cowpea mosaic virus (CPMV) virion RNA, a mutant has been isolated which is able to grow in Phaseolus vulgaris but is unable to grow in the ‘Early Red’ or ‘Blackeye Early Ramshorn’ varieties of cowpea (Vigna unguiculata). Dot blot analysis failed to detect virus-specific RNA in ‘Blackeye Early Ramshorn’ plants which had been inoculated with mutant 7A-4, indicating that no CPMV RNA synthesis had occurred. Supplementation experiments showed that the mutation was located in B RNA.

Keywords: CPMV, host range mutant, B RNA

Present address: Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, U.K.

Received 20 September 1984; accepted 31 October 1984.