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Lack of Sequence Homology between Double-stranded RNA from European and American Hypovirulent Strains of Endothia parasitica

¹ Department of Plant Pathology
and² Department of Biochemistry, University of Kentucky, Lexington, Kentucky 40546, U.S.A.

Dot blots of dsRNA from European and American hypovirulent (H) strains of the chestnut blight fungus, Endothia parasitica, were hybridized with ³²P-5'-end-labelled fragments of denatured dsRNA of French, Italian and American origins. Although dsRNA from European or American strains reacted well with labelled RNA probes from strains from the same continent there was little or no cross-hybridization between RNA from strains from different continents.

Keywords: dsRNA, hypovirulence, Endothia (Cryphonectria) parasitica, dot blot hybridization

Received 20 June 1984; accepted 16 October 1984.