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Mouse Interferon Alpha Beta Genes Are Linked at the Centromere Proximal Region of Chromosome 4

¹ Department of Pathology, Erasmus University, P.O. Box 1738, 3000 DR Rotterdam, The Netherlands
and² Division of Tumor Biology, The Netherlands Cancer Institute, (Antoni van Leeuwenhoek Huis), Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands

In order to determine the chromosomal localization of the murine interferon- (MuIFN-) and murine interferon- (MuIFN-) genes the DNAs of a panel of somatic cell hybrids were analysed by Southern blot hybridization. The hybrid cells were derived from E36 Chinese hamster cells and GRSL or GR MaTu mouse cells and retained all hamster chromosomes but segregated mouse chromosomes. The MuIFN- probe used was a 0.7 kb HindIII-EcoRI fragment derived from the MuIFN-₁ gene which hybridized with both mouse and hamster DNA. However, four fragments present in EcoRI digests of mouse DNA were clearly absent from the hybridization profile of EcoRI-digested hamster DNA and could be used for detection of MuIFN- sequences in the hybrid cells. The MuIFN- probe, a 0.5 kb BglII-BamHI fragment derived from the MuIFN- gene, hybridized with a 2.6 kb EcoRI fragment of mouse DNA and only weakly cross-hybridized with a 4.8 kb EcoRI fragment in hamster DNA. Southern blot analysis of DNA from mouse/hamster hybrids compared with the analysis of chromosome markers showed that both the MuIFN- and the MuIFN- genes are located on chromosome 4. Analysis of DNA from hybrids that contained only part of chromosome 4 indicated that the MuIFN- gene family and the MuIFN- gene are situated at the centromere-proximal region of the chromosome.

Keywords: MuIFN-, MuIFN-, chromosome 4

Received 20 September 1984; accepted 30 October 1984.

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